Genotoxic Evaluation of Furfuryl Alcohol and 2-Furyl Methyl Ketone by Sister Chromatid Exchange (SCE) Analysis
نویسندگان
چکیده
منابع مشابه
The sister chromatid exchange (SCE) assay.
A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for detecting alterations to the biochemistry underlying cellular homologous recombination. Growth of cells in the presence of 5'-bromo-deoxyuridine for t...
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Crystals of the title compound, C(12)H(10)N(4)O(5), were obtained from a condensation reaction of 2,4-dinitro-phenyl-hydrazine and 2-furyl methyl ketone. The mol-ecule displays a nearly planar structure, and the furan ring is slightly twisted by a dihedral angle of 12.62 (6)° with respect to the phenyl-hydrazone plane. The face-to-face separation of 3.287 (7) Å between parallel benzene rings of...
متن کاملSister chromatid exchange analysis in some Holstein bulls
Chromosomal appearance of 12 Holstein bulls selected for artificial inseminations were examined bysister chromatid exchanges (SCEs). For differential staining of sister chromatids bromodeoxyuridine (BrdU)was inoculated in lymphocyte cultures. The mean, maximum and minimum number of SCE per cell weredetermined 6.8 ± 1.14, 8.3 ± 1.1 and 5.7 ± 1.5, respectively. SCE frequencies of all animals were...
متن کاملSister Chromatid Exchange (sce) in Blood Lymphocytes of Bovine Leukaemia Virus Infected Cattle
The exchanges between sister chromatids were detected with the fluorescence plus Giemsa technique, in which the thymine analogue, bromodeoxyuridine, was used for DNA labelling. This method was used to examine the influence of bovine leukaemia virus on chromosomal disorders in bovine blood lymphocytes. In metaphasal chromosomes of BLV infected lymphocytes, numerous sister chromatid exchanges (SC...
متن کاملSister chromatid exchange in cancer.
Sister chromatid exchange (SCE) techniques have been shown to have great potential for use in the in vitro screening of suspected mutagens and carcinogens. Application of these techniques to bone marrow cells and/or lymphocytes from patients with various hematologic malignancies has demonstrated significant abnormalities in SCE frequencies in some of these diseases, as well as showing drug trea...
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ژورنال
عنوان ژورنال: Journal of Health Science
سال: 2007
ISSN: 1344-9702,1347-5207
DOI: 10.1248/jhs.53.124